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| Status |
Public on Jan 31, 2020 |
| Title |
6h_5_O2_rep1 |
| Sample type |
SRA |
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| Source name |
K562
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| Organism |
Homo sapiens |
| Characteristics |
cell line: K562
oxygen level: 5%
time point: 6hr
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| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNAeasy kit.
Picelli et al, 2014 (Smart-seq2 including PolyA selection)
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
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| Data processing |
Cufflinks suite (tophat, cuffquant, cuffnorm) as previously described (Trapnell et al., 2012)
Supplementary_files_format_and_content: tab-delimited text file contains FPKM values for each gene (row) for each sample (column)
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| Submission date |
Jan 30, 2020 |
| Last update date |
Feb 01, 2020 |
| Contact name |
Sarah E Calvo |
| E-mail(s) |
scalvo@broadinstitute.org
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| Organization name |
Broad Institute
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| Street address |
415 Main St
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| City |
Cambridge |
| State/province |
Massachusetts |
| ZIP/Postal code |
02141 |
| Country |
USA |
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| Platform ID |
GPL18573 |
| Series (1) |
| GSE144527 |
RNA-seq 6 day timecourse for K562 cells grown in hypoxia/normoxia (21%, 5%, 1% oxygen) |
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| Relations |
| BioSample |
SAMN13944750 |
| SRA |
SRX7650863 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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