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Status |
Public on Jan 31, 2020 |
Title |
24h_5_O2_rep1 |
Sample type |
SRA |
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Source name |
K562
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Organism |
Homo sapiens |
Characteristics |
cell line: K562 oxygen level: 5% time point: 24hr
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Extracted molecule |
total RNA |
Extraction protocol |
Qiagen RNAeasy kit. Picelli et al, 2014 (Smart-seq2 including PolyA selection)
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
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Data processing |
Cufflinks suite (tophat, cuffquant, cuffnorm) as previously described (Trapnell et al., 2012) Supplementary_files_format_and_content: tab-delimited text file contains FPKM values for each gene (row) for each sample (column)
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Submission date |
Jan 30, 2020 |
Last update date |
Feb 01, 2020 |
Contact name |
Sarah E Calvo |
E-mail(s) |
scalvo@broadinstitute.org
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Organization name |
Broad Institute
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Street address |
415 Main St
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City |
Cambridge |
State/province |
Massachusetts |
ZIP/Postal code |
02141 |
Country |
USA |
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Platform ID |
GPL18573 |
Series (1) |
GSE144527 |
RNA-seq 6 day timecourse for K562 cells grown in hypoxia/normoxia (21%, 5%, 1% oxygen) |
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Relations |
BioSample |
SAMN13944747 |
SRA |
SRX7650866 |
Supplementary data files not provided |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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