|
| Status |
Public on Jan 30, 2023 |
| Title |
Invasive ductal carcinoma 4 |
| Sample type |
RNA |
| |
|
| Source name |
RNA isolated from FFPE sections
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: breast
disease: Breast cancer
pathology: Invasive ductal carcinoma
further pathological characteristics: IHC showed homogeneous IDC
|
| Extracted molecule |
total RNA |
| Extraction protocol |
2-3 10 micrometer FFPE pathological slides were used to isolate RNA using a Qiagen miRNeasy FFPE kit
|
| Label |
biotin
|
| Label protocol |
Affymetrix FlashTag Biotin HSR RNA Labeling Kit
|
| |
|
| Hybridization protocol |
GeneChip® Cartridge Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 (Affymetrix).
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 7G.
|
| Data processing |
RMA-normalised expression was calculated using the R tool ‘oligo’ (Carvalho BS, Irizarry RA. “A Framework for Oligonucleotide Microarray Preprocessing” Bioinformatics (2010) 26(19), 2363-7).
|
| |
|
| Submission date |
Jan 30, 2020 |
| Last update date |
Jan 30, 2023 |
| Contact name |
Ernesto Yague |
| E-mail(s) |
ernesto.yague@imperial.ac.uk
|
| Organization name |
Imperial College London
|
| Department |
Division of Cancer
|
| Street address |
Du Cane Road
|
| City |
London |
| ZIP/Postal code |
W12 0NN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE144534 |
MicroRNA profile of breast cancer tissue samples |
|
