|
| Status |
Public on Jan 01, 2023 |
| Title |
TDP43WT-14w-TRAP-rep1 |
| Sample type |
SRA |
| |
|
| Source name |
Motor neurons from cervical spinal cord
|
| Organism |
Mus musculus |
| Characteristics |
strain: Chat bacTRAP line DW167 DW167Htz/J; TDP-43WT line 3cPtrc/J
disease state: Healthy control 2
cell type: Motor neurons
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Translating ribosome affinity purification (TRAP)
NEBNext UltraTM RNA Library Prep Kit for Illumina (New England Biolabs)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
STAR aligment
Counting by featureCounts
DESeq2 analysis
Genome_build: mm10
Supplementary_files_format_and_content: Rows represent genes
|
| |
|
| Submission date |
Jan 31, 2020 |
| Last update date |
Jan 01, 2023 |
| Contact name |
Kent Edward Duncan |
| Organization name |
University Medical Center, Hamburg-Eppendorf
|
| Department |
Center for Molecular Neurobiology
|
| Lab |
Duncan
|
| Street address |
Falkenried 94
|
| City |
Hamburg |
| ZIP/Postal code |
20251 |
| Country |
Germany |
| |
|
| Platform ID |
GPL21103 |
| Series (1) |
| GSE144640 |
TRAP-seq of spinal cord motor neurons from ALS diseased mice versus healthy mice |
|
| Relations |
| BioSample |
SAMN13961889 |
| SRA |
SRX7658574 |
