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Status |
Public on Jul 22, 2009 |
Title |
Rice wild-type panicle rep2 |
Sample type |
RNA |
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Source name |
rice wild-type panicle Rep2
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Organism |
Oryza sativa |
Characteristics |
genotype: Oryza sativa L. ssp. Indica, Zhongxian 3037 tissue: rice panicle (9cm-12cm)
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Growth protocol |
plants were grown in paddy fields in Beijing
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Extracted molecule |
total RNA |
Extraction protocol |
total RNA was isolated by TRIZOL and purified by Qiagen RNeasy kit.
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Label |
biotin
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Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 degree on GeneChip Rice Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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Scan protocol |
GeneChip was scanned using the Affymetrix GeneChip Scanner
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Description |
the panicle (9cm-12cm) was collected directly Gene expression data from rice mutant phoenix (pho) panicle
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Data processing |
The data were analyzed with GCOS software (Microarray Suite version 5.0, MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
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Submission date |
Jul 20, 2009 |
Last update date |
Aug 06, 2009 |
Contact name |
Wenying Xu |
E-mail(s) |
wyxu@genetics.ac.cn
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Organization name |
Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences
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Street address |
Datun Road, Chaoyang District
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City |
Beijing |
ZIP/Postal code |
100101 |
Country |
China |
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Platform ID |
GPL2025 |
Series (1) |
GSE17194 |
Genome-wide gene expression profiling of rice Indica cultivar Zhongxian 3037 and mutant phoenix (pho) panicle |
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