 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Feb 11, 2020 |
| Title |
CC1 |
| Sample type |
RNA |
| |
|
| Source name |
Compound C
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Primary human hepatocytes
activation agent: COMPOUND C
time: 24h treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with RNeasy Mini Kit and retrotranscribed using Megaplex™ RT Primers and TaqMan™ MicroRNA Reverse Transcription Kit RT Primers, TaqMan™ MicroRNA Reverse Transcription Kit
|
| Label |
TaqMan
|
| Label protocol |
PCR assays were performed using the TaqMan™ Array Human MicroRNA A+B Cards Set v3.0 (ThermoFisher Scientific, Waltham, Massachusetts, USA). Catalog # 4444913.
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
Test 2
|
| Data processing |
The normalization was performed using the raw Ct value-average of raw Cts for all miRNAs with reliable results
Target miRNA signals normalized to the average; 2^-deltaCt, where deltaCt = (Ct_Target − Ct_miRNA mean)]
The web-based software package gives the raw threshold cycle data.
Fold Change data file reports test/control (i.e., palmitate/BSA) ratios.
Normalized data file includes normalized signal (against the average of all miRNAs detected).
Fold Change worksheet reports test/control (i.e., palmitate/BSA) ratios.
|
| |
|
| Submission date |
Feb 10, 2020 |
| Last update date |
Feb 11, 2020 |
| Contact name |
Jessica Latorre |
| E-mail(s) |
jlatorre@idibgi.org
|
| Phone |
655368990
|
| Organization name |
IDIBGI
|
| Street address |
C/ Dr Castany s/n
|
| City |
Salt |
| ZIP/Postal code |
17190 |
| Country |
Spain |
| |
|
| Platform ID |
GPL18942 |
| Series (1) |
| GSE145039 |
Real-time quantitative PCR miRNA analysis of human hepatocytes |
|
| Supplementary data files not provided |
| Processed data are available on Series record |
|
|
|
|
 |
