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| Status |
Public on Feb 11, 2020 |
| Title |
CMET1 |
| Sample type |
RNA |
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| Source name |
Control Metformin
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| Organism |
Homo sapiens |
| Characteristics |
cell type: Primary human hepatocytes
activation agent: PBS
time: 24h treatment
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with RNeasy Mini Kit and retrotranscribed using Megaplex™ RT Primers and TaqMan™ MicroRNA Reverse Transcription Kit RT Primers, TaqMan™ MicroRNA Reverse Transcription Kit
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| Label |
TaqMan
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| Label protocol |
PCR assays were performed using the TaqMan™ Array Human MicroRNA A+B Cards Set v3.0 (ThermoFisher Scientific, Waltham, Massachusetts, USA). Catalog # 4444913.
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| Hybridization protocol |
n/a
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| Scan protocol |
n/a
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| Description |
Control 3
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| Data processing |
The normalization was performed using the raw Ct value-average of raw Cts for all miRNAs with reliable results
Target miRNA signals normalized to the average; 2^-deltaCt, where deltaCt = (Ct_Target − Ct_miRNA mean)]
The web-based software package gives the raw threshold cycle data.
Fold Change data file reports test/control (i.e., palmitate/BSA) ratios.
Normalized data file includes normalized signal (against the average of all miRNAs detected).
Fold Change worksheet reports test/control (i.e., palmitate/BSA) ratios.
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| Submission date |
Feb 10, 2020 |
| Last update date |
Feb 11, 2020 |
| Contact name |
Jessica Latorre |
| E-mail(s) |
jlatorre@idibgi.org
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| Phone |
655368990
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| Organization name |
IDIBGI
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| Street address |
C/ Dr Castany s/n
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| City |
Salt |
| ZIP/Postal code |
17190 |
| Country |
Spain |
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| Platform ID |
GPL18942 |
| Series (1) |
| GSE145039 |
Real-time quantitative PCR miRNA analysis of human hepatocytes |
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| Supplementary data files not provided |
| Processed data are available on Series record |
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