|
| Status |
Public on Dec 20, 2020 |
| Title |
PreB_RNAseq_shRen_4 |
| Sample type |
SRA |
| |
|
| Source name |
PreB cells
|
| Organism |
Mus musculus |
| Characteristics |
tissue: PreB cells
plasmids: (MSCV-shRNA-SV40-Myc-IRES-GFP)eMLM-shRen
replicates: shRen_rep3
|
| Treatment protocol |
24h after isolation and culture, preB cells were transducted with eMLM-shRen or eMLM-shPhf23 through retroviral infection. 48h after infection, the transducted cells labelled with GFP were sorted out by FACS AriaIII. Immediately after sorting, the cells were centrifugated to obtain cell pellets, followed by Trizol addition.
|
| Growth protocol |
preB cells were cultured in RPMI 1640 medium supplemented with 20% gibco FBS, 1% Pen/Strep and 10ng/ml mouse IL7.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was extracted with Trizol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
| |
|
| Data processing |
Sequenced reads were mapped to GRCm38 whole genome using STAR_2.6.0a
Counts were normalized by Transcript per million (TPM)
processed data files format and content: txt files with gene TPM
|
| |
|
| Submission date |
Feb 12, 2020 |
| Last update date |
Dec 20, 2020 |
| Contact name |
Xaingyu Pan |
| E-mail(s) |
pangxueyu233@outlook.com
|
| Organization name |
Sichuan University
|
| Department |
State Key Laboratory of Biotherapy, West China Hospital, Sihcuan University.
|
| Lab |
Chen & Liu Lab
|
| Street address |
No.17 Renming road section3
|
| City |
Chengdu, Cihna |
| State/province |
Sichuan |
| ZIP/Postal code |
610041 |
| Country |
China |
| |
|
| Platform ID |
GPL23479 |
| Series (1) |
| GSE145190 |
An epigenetic mechanism underlying chromosome 17p deletion-driven tumorigenesis |
|
| Relations |
| BioSample |
SAMN14088608 |
| SRA |
SRX7713730 |
