|
Status |
Public on Jul 01, 2021 |
Title |
InR-DN 1 oocyte |
Sample type |
SRA |
|
|
Source name |
stage 14 oocytes
|
Organism |
Drosophila melanogaster |
Characteristics |
genotype: InR-DN
|
Growth protocol |
flies were grown on standard bottle food supplemented with yeast paste
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated from sample of 200 stage 14 oocytes using tripure RNA isolation reagent TruSeq Stranded Total RNA LT Sample Prep Kit from Illumina
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Description |
polyA selected InR-DN progeny
|
Data processing |
baseliing using a HiSeq 2500 QC control: FASTQC report with basic sequencing quality statistics mapping reads : Bowtie tophat differential analysis: Edge R Genome_build: Drosophila release 6 Supplementary_files_format_and_content: igenomes_known_GENES_counts_con_embryos-VS-InR_embryos.glmLRT.txt
|
|
|
Submission date |
Feb 14, 2020 |
Last update date |
Jul 01, 2021 |
Contact name |
Matt Sieber |
E-mail(s) |
matthew.sieber@utsouthwestern.edu
|
Phone |
2146456036
|
Organization name |
UT Southwestern Medical Center
|
Street address |
5323 Harry Hines blvd, MC 9040 ND12.214
|
City |
Dallas |
State/province |
Texas |
ZIP/Postal code |
75390 |
Country |
USA |
|
|
Platform ID |
GPL17275 |
Series (2) |
GSE145351 |
Examine the impact of insulin suppression on oocyte gene expression. |
GSE145353 |
Examine the impact of insulin suppression on oocyte and embryo gene expression |
|
Relations |
BioSample |
SAMN14114054 |
SRA |
SRX7730337 |