|
| Status |
Public on Jul 01, 2021 |
| Title |
InR-DN 3 oocyte |
| Sample type |
SRA |
| |
|
| Source name |
stage 14 oocytes
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: InR-DN
|
| Growth protocol |
flies were grown on standard bottle food supplemented with yeast paste
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from sample of 200 stage 14 oocytes using tripure RNA isolation reagent
TruSeq Stranded Total RNA LT Sample Prep Kit from Illumina
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
polyA selected
InR-DN progeny
|
| Data processing |
baseliing using a HiSeq 2500
QC control: FASTQC report with basic sequencing quality statistics
mapping reads : Bowtie
tophat
differential analysis: Edge R
Genome_build: Drosophila release 6
Supplementary_files_format_and_content: igenomes_known_GENES_counts_con_embryos-VS-InR_embryos.glmLRT.txt
|
| |
|
| Submission date |
Feb 14, 2020 |
| Last update date |
Jul 01, 2021 |
| Contact name |
Matt Sieber |
| E-mail(s) |
matthew.sieber@utsouthwestern.edu
|
| Phone |
2146456036
|
| Organization name |
UT Southwestern Medical Center
|
| Street address |
5323 Harry Hines blvd, MC 9040 ND12.214
|
| City |
Dallas |
| State/province |
Texas |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL17275 |
| Series (2) |
| GSE145351 |
Examine the impact of insulin suppression on oocyte gene expression. |
| GSE145353 |
Examine the impact of insulin suppression on oocyte and embryo gene expression |
|
| Relations |
| BioSample |
SAMN14114056 |
| SRA |
SRX7730339 |
