|
| Status |
Public on Apr 20, 2020 |
| Title |
DIF P11 D |
| Sample type |
SRA |
| |
|
| Source name |
frozen mycelia
|
| Organism |
Penicillium expansum |
| Characteristics |
treatment: exposed to difenoconazole
|
| Treatment protocol |
5ppm difenoconazole addition to liquid shake culture for samples beginning with "D" acetone for samples beginning with "A"
|
| Growth protocol |
biological Replicates are deliniated alphabetically with the letter after M in sample name.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
trizol with bead beating in qiagen tissue lyzer
Libraries were prepared according to Illumina's instructions accompanying the TruSeq Stranded mRNA Library Prep Kit.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
FAST-QC v. 0.11.9
Trinity de novo Alignment to reference (MD-8 genome) usegalaxy.org
Salmon transcriptome read counting usegalaxy.org
Genome_build: reference genome: GCA_000769745.1
Supplementary_files_format_and_content: gene count data from transcriptome as aligned to reference genome
|
| |
|
| Submission date |
Feb 14, 2020 |
| Last update date |
Apr 21, 2020 |
| Contact name |
Wayne M Jurick |
| E-mail(s) |
Wayne.jurick@usda.gov
|
| Organization name |
United States Department of Agriculture
|
| Department |
Agricultural Research Service
|
| Lab |
Food Quality
|
| Street address |
Building 002
|
| City |
Beltsville |
| State/province |
MD |
| ZIP/Postal code |
20705 |
| Country |
USA |
| |
|
| Platform ID |
GPL28150 |
| Series (1) |
| GSE145355 |
Fungicide resistance RNA-seq on two isolates of Penicillium |
|
| Relations |
| BioSample |
SAMN14114306 |
| SRA |
SRX7730356 |
