|
Status |
Public on Apr 20, 2020 |
Title |
Acetone G10 D |
Sample type |
SRA |
|
|
Source name |
frozen mycelia
|
Organism |
Penicillium crustosum |
Characteristics |
treatment: exposed to acetone
|
Treatment protocol |
5ppm difenoconazole addition to liquid shake culture for samples beginning with "D" acetone for samples beginning with "A"
|
Growth protocol |
biological Replicates are deliniated alphabetically with the letter after M in sample name.
|
Extracted molecule |
total RNA |
Extraction protocol |
trizol with bead beating in qiagen tissue lyzer Libraries were prepared according to Illumina's instructions accompanying the TruSeq Stranded mRNA Library Prep Kit.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Data processing |
FAST-QC v. 0.11.9 Trinity de novo Alignment to reference (MD-8 genome) usegalaxy.org Salmon transcriptome read counting usegalaxy.org Genome_build: reference genome: GCA_000769745.1 Supplementary_files_format_and_content: gene count data from transcriptome as aligned to reference genome
|
|
|
Submission date |
Feb 14, 2020 |
Last update date |
Apr 21, 2020 |
Contact name |
Wayne M Jurick |
E-mail(s) |
Wayne.jurick@usda.gov
|
Organization name |
United States Department of Agriculture
|
Department |
Agricultural Research Service
|
Lab |
Food Quality
|
Street address |
Building 002
|
City |
Beltsville |
State/province |
MD |
ZIP/Postal code |
20705 |
Country |
USA |
|
|
Platform ID |
GPL28151 |
Series (1) |
GSE145355 |
Fungicide resistance RNA-seq on two isolates of Penicillium |
|
Relations |
BioSample |
SAMN14114294 |
SRA |
SRX7730368 |