|
| Status |
Public on Mar 01, 2021 |
| Title |
day2 rep 3 |
| Sample type |
SRA |
| |
|
| Source name |
Cell culture
|
| Organism |
Mus musculus |
| Characteristics |
strain: 3T3-L1 preadipocytes
|
| Treatment protocol |
Insulin-induced differentiation and MCH treatment
|
| Growth protocol |
Cell line standard culture protocol based on ATCC
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extracted using Rneasy Mini Kit(Qiagen)
WaferGen PrepXTM mRNA Strand-Specific Library Preparation Protocol (WaferGen Biosystems)
Illumina mulitplex indexed single-end
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Data processing |
alignment by STAR v2.5.2a
Reads count by HTSEQ v0.11.2
filtering out low-expressed genes
DESeq2 v1.24.0
Genome_build: mm10
Supplementary_files_format_and_content: reads count data, Excel .xlsx file format
|
| |
|
| Submission date |
Feb 18, 2020 |
| Last update date |
Mar 01, 2021 |
| Contact name |
Rongkun Shen |
| Organization name |
SUNY Brockport
|
| Department |
Biology
|
| Street address |
350 New Campus Dr
|
| City |
Brockport |
| State/province |
New York |
| ZIP/Postal code |
14420-2997 |
| Country |
USA |
| |
|
| Platform ID |
GPL21493 |
| Series (1) |
| GSE145452 |
Transcriptome Analysis of Ciliary-Dependent MCH Signaling in Differentiating 3T3-L1 Preadipocytes |
|
| Relations |
| BioSample |
SAMN14124655 |
| SRA |
SRX7739454 |
