|
| Status |
Public on Jul 31, 2009 |
| Title |
anr mutant exponential #2 |
| Sample type |
RNA |
| |
|
| Source name |
strain PAO6261 (anr), exponential phase #2
|
| Organism |
Pseudomonas aeruginosa |
| Characteristics |
growth phase: exponential phase #2
genotype: anr mutant
|
| Treatment protocol |
The culture was mixed with 2 volumes of RNAprotect Bacterial Reagent (Qiagen) before RNA purification.
|
| Growth protocol |
P. aeruginosa strains were cultivated aerobically in LB medium in Erlenmeyer flasks. Total RNA was extracted when optical density at 600 nm was 0.3.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by a hot-phenol method. After contaminated DNA was eliminated by RQ1 DNase (Promega) treatment, RNA was purified by RNeasy mini columns (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Enzo BioArray terminal labeling kit
|
| |
|
| Hybridization protocol |
Target hybridization, staining, and scanning were performed according to the instructions designed for the Pseudomonas aeruginosa genome array by Affymetrix.
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 was used.
|
| Description |
GeneChip Hybridization Oven 640 and Fluidics Station 450 were used for target hybridization and staining under the control of the Affymetrix GeneChip Operating Software.
|
| Data processing |
The Affymetrix GeneChip Operating Software was used for data acquisition and processing.
|
| |
|
| Submission date |
Jul 24, 2009 |
| Last update date |
Jul 27, 2009 |
| Contact name |
Hiroyuki Arai |
| Organization name |
The University of Tokyo
|
| Department |
Department of Biotechnology
|
| Lab |
Lab. of Applied Microbiology
|
| Street address |
Yayoi 1-1-1
|
| City |
Bunkyo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
113-8657 |
| Country |
Japan |
| |
|
| Platform ID |
GPL84 |
| Series (1) |
| GSE17296 |
Transcriptome analysis of the roxSR and anr mutant strains of Pseudomonas aeruginosa under aerobic conditions |
|
