|
Status |
Public on Jul 31, 2009 |
Title |
roxSR mutant early stationary #1 |
Sample type |
RNA |
|
|
Source name |
strain ROX1 (roxSR), early stationary phase #1
|
Organism |
Pseudomonas aeruginosa |
Characteristics |
growth phase: early stationary phase #1 genotype: roxSR mutant
|
Treatment protocol |
The culture was mixed with 2 volumes of RNAprotect Bacterial Reagent (Qiagen) before RNA purification.
|
Growth protocol |
P. aeruginosa strains were cultivated aerobically in LB medium in Erlenmeyer flasks. Total RNA was extracted when optical density at 600 nm was 1.4.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted by a hot-phenol method. After contaminated DNA was eliminated by RQ1 DNase (Promega) treatment, RNA was purified by RNeasy mini columns (Qiagen).
|
Label |
biotin
|
Label protocol |
Enzo BioArray terminal labeling kit
|
|
|
Hybridization protocol |
Target hybridization, staining, and scanning were performed according to the instructions designed for the Pseudomonas aeruginosa genome array by Affymetrix.
|
Scan protocol |
Affymetrix GeneChip Scanner 3000 was used.
|
Description |
GeneChip Hybridization Oven 640 and Fluidics Station 450 were used for target hybridization and staining under the control of the Affymetrix GeneChip Operating Software.
|
Data processing |
The Affymetrix GeneChip Operating Software was used for data acquisition and processing.
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|
|
Submission date |
Jul 24, 2009 |
Last update date |
Jul 27, 2009 |
Contact name |
Hiroyuki Arai |
Organization name |
The University of Tokyo
|
Department |
Department of Biotechnology
|
Lab |
Lab. of Applied Microbiology
|
Street address |
Yayoi 1-1-1
|
City |
Bunkyo-ku |
State/province |
Tokyo |
ZIP/Postal code |
113-8657 |
Country |
Japan |
|
|
Platform ID |
GPL84 |
Series (1) |
GSE17296 |
Transcriptome analysis of the roxSR and anr mutant strains of Pseudomonas aeruginosa under aerobic conditions |
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