mRNA purification, cDNA synthesis, cRNA preparation, and hybridization to the Human Genome U133Plus 2.0 GeneChip microarrays (Affymetrix, Santa Clara, CA) were performed as previously described in PMID: 17105813
Label
biotin
Label protocol
mRNA purification, cDNA synthesis, cRNA preparation, and hybridization to the Human Genome U133Plus 2.0 GeneChip microarrays (Affymetrix, Santa Clara, CA) were performed as previously described PMID: 17105813
Hybridization protocol
mRNA purification, cDNA synthesis, cRNA preparation, and hybridization to the Human Genome U133Plus 2.0 GeneChip microarrays (Affymetrix, Santa Clara, CA) were performed as previously described PMID: 17105814
Scan protocol
mRNA purification, cDNA synthesis, cRNA preparation, and hybridization to the Human Genome U133Plus 2.0 GeneChip microarrays (Affymetrix, Santa Clara, CA) were performed as previously described PMID: 17105815
Description
Gene expression data from CD138+ selected plasma cells
Data processing
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
