|
| Status |
Public on Jul 01, 2015 |
| Title |
PC 81 TE |
| Sample type |
RNA |
| |
|
| Source name |
Prostate Biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
Tumor Epithelial
Batch 3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen miRNeasy (miR+mRNA)
|
| Label |
Hy3
|
| Label protocol |
Refer to miRCURY LNA (TM) microRNA Array Power Labeling Kit
|
| |
|
| Hybridization protocol |
Refer to miRCURY LNA (TM) microRNA Array microarray kit instruction manual v9.2
(manual procedure)
|
| Scan protocol |
Scanner=GenePix 4000B [92209]
PixelSize=10
BackgroundSubtraction=LocalFeature
PMTGain=650
Genepix software package was used to generate an intensity value for each probe by taking the median spot intensity and subtracting the median local background. Bad spots or areas of the array with obvious defects were manually flagged. Flagged spots were not included in subsequent analyses.
|
| Description |
N/A
|
| Data processing |
The hsa_negative probes were averaged for each array and used as a threshold value. The array's threshold was subtracted from all of the array's averaged discovery probes. PC73 TNE was the overlapping standard run in each of the six batches. Comparison of the overlapping sample was used to carry out an inter-batch normalization.
|
| |
|
| Submission date |
Jul 24, 2009 |
| Last update date |
Jul 01, 2015 |
| Contact name |
John Michael Krill-Burger |
| E-mail(s) |
burgerm@upmc.edu
|
| Phone |
412-656-6727
|
| Organization name |
University of Pittsburgh Medical Center
|
| Street address |
Rm. WG21.3 Shadyside Hospital
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15232 |
| Country |
USA |
| |
|
| Platform ID |
GPL7724 |
| Series (1) |
| GSE17321 |
miRNA Expression in 4 prostate cell types from human tumor biopsies |
|
