|
| Status |
Public on Sep 02, 2021 |
| Title |
CLL583_mRNA |
| Sample type |
RNA |
| |
|
| Source name |
CD19+ B cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Peripheral blood
disease state: chronic lymphocytic leukemia
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was purified using Qiagen RNAeasy Kits according to the manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
Purified RNA was amplified using Nugen Ovation Pico SL or Ovation Pico kits and labeled using Nugen Encore Biotin kit
|
| |
|
| Hybridization protocol |
Products were hybridized to Affymetrix Human Gene 2.0 ST arrays according to the manufacturer's protocol
|
| Scan protocol |
GS3000
|
| Data processing |
Gene expression levels were determined using the Applied Biosystems Transcriptome Analysis Console (v4.0.2.15) using rma normalization, HuGene2.0.na36 annotations, and default settings.
probe group file: HuGene-2_0-st.pgf
meta-probeset file: HuGene-2_0-st-v1.na36.transcript.csv
|
| |
|
| Submission date |
Feb 24, 2020 |
| Last update date |
Sep 02, 2021 |
| Contact name |
Jared Andrews |
| E-mail(s) |
jared.andrews@stjude.org
|
| Organization name |
St. Jude Children's Research Hospital
|
| Department |
Developmental Neurobiology
|
| Street address |
262 Danny Thomas Pl
|
| City |
Memphis |
| State/province |
Tennessee |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL16686 |
| Series (2) |
| GSE145847 |
Key Super Enhancers Drive Tumor-Suppressing Transcription Feedback Programs in Mature B Cell Cancers (RNA array) |
| GSE145848 |
Key Super Enhancers Drive Tumor-Suppressing Transcription Feedback Programs in Mature B Cell Cancers |
|
