|
| Status |
Public on Dec 31, 2020 |
| Title |
Longissimus_Genmascq_Pig_143 |
| Sample type |
RNA |
| |
|
| Source name |
Longissimus lumborum muscle
|
| Organism |
Sus scrofa |
| Characteristics |
gender: M
pig number: 143
ChIP: 27
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Total RNA (500 ng) from each animal was labeled individually with Cy3 by using the Quick-Amp Labeling Kit following the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Microarray hybridizations were carried out in Agilent’s SureHyb Hybridization Chambers containing 300 ng of cyanine 3-labeled cRNA sample per hybridization. The hybridization reactions were performed at 65°C for 17 hours using Agilent’s Gene Expression Hybridization Kit. Slides were disassembled and washed for 1 minute at room temperature in SSPE wash solution 1 (6x SSPE + 0.005% Sarcosine) and in SSPE wash solution 2 (0.06x SSPE + 0.005% Sarcosine) for one minute at room temperature. The microarrays were then treated with Agilent Stabilization and Drying Solution at room temperature for 30 seconds.
|
| Scan protocol |
Scanned on an Agilent G2505B scanner. Images were quantified using Agilent Feature Extraction Software (version 9.5).
|
| Data processing |
Raw data from 15198 probes fluorescent hybridization signal intensities were log transformed and centered within sample by subtraction of sample median from all raw values.
|
| |
|
| Submission date |
Feb 25, 2020 |
| Last update date |
Jan 01, 2021 |
| Contact name |
Frederic Herault |
| E-mail(s) |
frederic.herault@inrae.fr
|
| Phone |
33 2 23 48 50 45
|
| Organization name |
INRAE
|
| Department |
Animal Genetic
|
| Street address |
16 le Clos
|
| City |
SAINT GILLES |
| ZIP/Postal code |
35590 |
| Country |
France |
| |
|
| Platform ID |
GPL11445 |
| Series (1) |
| GSE145892 |
Gene expression in Longissimus lumborum skeletal muscles in pig using the GenmascqChip (custom 15K Agilent microarray) |
|
