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Sample GSM434055 Query DataSets for GSM434055
Status Public on Feb 02, 2011
Title small airways, smoker 247
Sample type RNA
 
Source name airway epithelial cells obtained by bronchoscopy and brushing
Organism Homo sapiens
Characteristics age: 47
sex: M
ethnic group: black
smoking status: smoker, 19.5 pack-years
Extracted molecule total RNA
Extraction protocol Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-2 microg total RNA (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 7G.
Description no additional information
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Jul 28, 2009
Last update date Feb 02, 2011
Contact name Yael Strulovici-Barel
E-mail(s) yas2003@med.cornell.edu
Organization name Weill Cornell Medical College
Department Department of Genetic Medicine
Lab Crystal
Street address 1300 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platform ID GPL570
Series (7)
GSE11784 Biologic Phenotyping of the Human Small Airway Epithelial Response to Cigarette Smoking
GSE17905 Smoking-mediated Up-regulation of GAD67 Expression in the Human Airway Epithelium
GSE18385 Smoking-induced Up-regulation of AKR1B10 Expression in the Airway Epithelium of Healthy Individuals

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 1172.32 P 7.00668e-05
AFFX-BioB-M_at 2119.75 P 4.42873e-05
AFFX-BioB-3_at 1114.36 P 4.42873e-05
AFFX-BioC-5_at 3981.41 P 8.14279e-05
AFFX-BioC-3_at 5545.49 P 4.42873e-05
AFFX-BioDn-5_at 8625.67 P 4.42873e-05
AFFX-BioDn-3_at 14297.2 P 6.02111e-05
AFFX-CreX-5_at 56438.2 P 5.16732e-05
AFFX-CreX-3_at 57691.8 P 4.42873e-05
AFFX-DapX-5_at 74.2266 A 0.0726824
AFFX-DapX-M_at 17.2254 A 0.672921
AFFX-DapX-3_at 24.2634 A 0.631562
AFFX-LysX-5_at 22.9771 A 0.440646
AFFX-LysX-M_at 4.2722 A 0.937087
AFFX-LysX-3_at 6.57557 A 0.544587
AFFX-PheX-5_at 38.8634 A 0.38256
AFFX-PheX-M_at 4.24232 A 0.971543
AFFX-PheX-3_at 51.0439 A 0.425962
AFFX-ThrX-5_at 33.5975 A 0.58862
AFFX-ThrX-M_at 30.3339 A 0.41138

Total number of rows: 54675

Table truncated, full table size 1639 Kbytes.




Supplementary file Size Download File type/resource
GSM434055.CEL.gz 8.0 Mb (ftp)(http) CEL
GSM434055.CHP.gz 13.1 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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