|
| Status |
Public on Aug 05, 2009 |
| Title |
Mouse astrocytes exposed to mock virus rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Primary mouse astrocytes, mock
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: primary astrocytes
age: neonatal day 2
treatment: mock virus
|
| Biomaterial provider |
The Jackson Laboratory (Bar Harbor, ME)
|
| Treatment protocol |
Day-two neonatal mouse astrocytes were exposed to mock virus at m.o.i. of 1 and cultured for 24 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared from cell cultures using the RNAeasy total RNA extraction kit (Qiagen, CA). RNA quality was assessed by electrophoresis and spectrophotometric analysis.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
Mouse astrocytes exposed to mock virus rep2
|
| Data processing |
CHP files were obtained using the Affymetrix software, MAS5 algorithm.
|
| |
|
| Submission date |
Jul 28, 2009 |
| Last update date |
Aug 04, 2009 |
| Contact name |
Alejandra Borjabad |
| E-mail(s) |
ab2612@columbia.edu
|
| Phone |
9175574628
|
| Organization name |
St.Luke's-Roosevelt Hospital Center
|
| Department |
Medicine
|
| Lab |
Molecular Virology
|
| Street address |
432 West 58th, Street
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10019 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE17383 |
Toward a Better Understanding of Potential Roles of Astrocytes in HIV-1-associated Neurocognitive Disorders |
|
