|
Status |
Public on Aug 05, 2009 |
Title |
Mouse astrocytes exposed to mock virus rep2 |
Sample type |
RNA |
|
|
Source name |
Primary mouse astrocytes, mock
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 cell type: primary astrocytes age: neonatal day 2 treatment: mock virus
|
Biomaterial provider |
The Jackson Laboratory (Bar Harbor, ME)
|
Treatment protocol |
Day-two neonatal mouse astrocytes were exposed to mock virus at m.o.i. of 1 and cultured for 24 hours.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was prepared from cell cultures using the RNAeasy total RNA extraction kit (Qiagen, CA). RNA quality was assessed by electrophoresis and spectrophotometric analysis.
|
Label |
biotin
|
Label protocol |
Standard Affymetrix protocol
|
|
|
Hybridization protocol |
Standard Affymetrix protocol
|
Scan protocol |
Standard Affymetrix protocol
|
Description |
Mouse astrocytes exposed to mock virus rep2
|
Data processing |
CHP files were obtained using the Affymetrix software, MAS5 algorithm.
|
|
|
Submission date |
Jul 28, 2009 |
Last update date |
Aug 04, 2009 |
Contact name |
Alejandra Borjabad |
E-mail(s) |
ab2612@columbia.edu
|
Phone |
9175574628
|
Organization name |
St.Luke's-Roosevelt Hospital Center
|
Department |
Medicine
|
Lab |
Molecular Virology
|
Street address |
432 West 58th, Street
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10019 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE17383 |
Toward a Better Understanding of Potential Roles of Astrocytes in HIV-1-associated Neurocognitive Disorders |
|