|
| Status |
Public on Sep 01, 2009 |
| Title |
Satb1-/- clone 1-1 |
| Sample type |
RNA |
| |
|
| Source name |
murine Satb1-/- ES cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: embryonic stem cells
genotype/variation: Satb1 knockout
|
| Treatment protocol |
Cells were grown for 3 days on irradiated feeders after trypsinisation and cultured in the presence of hygromycin
|
| Growth protocol |
Cells were passaged according to standard procedures with standard ES medium (Non essential AA, Pyruvate, PSG, LIF, 15% serum, beta-mercaptoethanol)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After 3 days, cells were trypsinized, resuspended in 15ml of medium, allowed to settle for 10 minutes to eliminate feeders. The top 50% of the suspension was used for RNA preparation/Trizol.
|
| Label |
biotin
|
| Label protocol |
Performed by Genomics Core Facility, EMBL Heidelberg
|
| |
|
| Hybridization protocol |
Performed by Genomics Core Facility, EMBL Heidelberg
|
| Scan protocol |
Performed by Genomics Core Facility, EMBL Heidelberg
|
| Description |
Gene expression data from murine Satb -/- ES cells
|
| Data processing |
The data were analyzed using the RMA method of background correction, quantile normalization, and summarization of cell signal intensity
|
| |
|
| Submission date |
Aug 03, 2009 |
| Last update date |
Aug 03, 2009 |
| Contact name |
Rudolf Engelke |
| E-mail(s) |
engelke@immunbio.mpg.de
|
| Phone |
+49 761 5108 751
|
| Organization name |
Max Planck Institute of Immunobiology
|
| Street address |
Stuebeweg 51
|
| City |
Freiburg |
| ZIP/Postal code |
79108 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE17487 |
Expression data in WT and Satb1-/- ES cells |
| GSE17490 |
Role of Satb1 and Satb2 in embryonic stem cells |
|
