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Sample GSM436113 Query DataSets for GSM436113
Status Public on Sep 01, 2009
Title tetO-Satb1 #2
Sample type RNA
 
Source name murine ES cells
Organism Mus musculus
Characteristics cell type: embryonic stem cells
genotype/variation: control
Treatment protocol Cells were grown for 1 days on irradiated feeders after trypsinisation and either cultured for 1 more day under these conditions or induced for 24h by the addition of DOX.
Growth protocol Cells were passaged according to standard procedures with standard ES medium (Non essential AA, Pyruvate, PSG, LIF, 15% serum, beta-mercaptoethanol)
Extracted molecule total RNA
Extraction protocol After 2 days of culture, cells were trypsinized, resuspended in 15ml of medium, allowed to settle for 10 minutes to eliminate feeders. The top 50% of the suspension was used for RNA preparation/Trizol.
Label biotin
Label protocol Performed by Genomics Core Facility, EMBL Heidelberg
 
Hybridization protocol Performed by Genomics Core Facility, EMBL Heidelberg
Scan protocol Performed by Genomics Core Facility, EMBL Heidelberg
Description Gene expression data from murine ES cells
Data processing The data were analyzed using the RMA method of background correction, quantile normalization, and summarization of cell signal intensity
 
Submission date Aug 03, 2009
Last update date Aug 03, 2009
Contact name Rudolf Engelke
E-mail(s) engelke@immunbio.mpg.de
Phone +49 761 5108 751
Organization name Max Planck Institute of Immunobiology
Street address Stuebeweg 51
City Freiburg
ZIP/Postal code 79108
Country Germany
 
Platform ID GPL6246
Series (2)
GSE17488 Expression data in WT and ES cells overexpressing Satb1
GSE17490 Role of Satb1 and Satb2 in embryonic stem cells

Data table header descriptions
ID_REF
VALUE RMA cell signal intensity

Data table
ID_REF VALUE
10338001 10.621359
10338002 5.073656
10338003 9.0020075
10338004 7.763962
10338005 1.4133226
10338006 1.5084529
10338007 1.8048316
10338008 2.4965954
10338009 6.6659193
10338010 1.3956116
10338011 4.4083095
10338012 1.4133226
10338013 1.304493
10338014 1.3265849
10338015 1.3265849
10338016 6.3951964
10338017 11.769806
10338018 5.460119
10338019 4.094092
10338020 6.823511

Total number of rows: 35557

Table truncated, full table size 643 Kbytes.




Supplementary file Size Download File type/resource
GSM436113.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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