|
| Status |
Public on Nov 14, 2009 |
| Title |
MCC Patient 74 |
| Sample type |
RNA |
| |
|
| Source name |
Moffitt Cancer Center
|
| Organism |
Homo sapiens |
| Characteristics |
age: 51
gender: male
ethnicity: caucasian
ajcc_stage: 2
grade: 2 - Moderately differentiated (MD)
overall_event (death from any cause): no death
dss_event (disease specific survival; death from cancer): no death
dfs_event (disease free survival; cancer recurrence): no recurrence
overall survival follow-up time: 33.2
dss_time: 33.2
dfs_time: 33.2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Qiagen RNeasy kit.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocols were used.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocols for hybridization to the Affy HGU133 2.0 plus array were used.
|
| Scan protocol |
GeneChip Scanner was used for scanning and generating the raw .CEL files.
|
| Description |
Gene expression
|
| Data processing |
Bioconductor's affy package was used for RMA normalization and raw data processing using default settings for background correction and normalization. Cox regression hazards model was applied to the processed data using the survival package. All analyses were performed using R software.
|
| |
|
| Submission date |
Aug 06, 2009 |
| Last update date |
Nov 02, 2009 |
| Contact name |
Pengcheng Lu |
| E-mail(s) |
pengcheng.lu@vanderbilt.edu
|
| Organization name |
Vanderbilt University
|
| Street address |
2200 Pierce Avenue
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE17536 |
Metastasis Gene Expression Profile Predicts Recurrence and Death in Colon Cancer Patients (Moffitt Samples) |
| GSE17538 |
Experimentally Derived Metastasis Gene Expression Profile Predicts Recurrence and Death in Colon Cancer Patients |
|
