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| Status |
Public on Aug 13, 2009 |
| Title |
Brain Cerebellum Pool_10 |
| Sample type |
RNA |
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| Source name |
BD Clontech – Brain Cerebellum polyA RNA
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| Organism |
Homo sapiens |
| Characteristics |
tissue: cerebellum
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| Growth protocol |
11 tissues poly A+ RNA was purchased and 5 cell lines were grown as suggested by ATCC
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| Extracted molecule |
polyA RNA |
| Extraction protocol |
see Kapranov, et al Science 2002
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| Label |
Biotin
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| Label protocol |
2 micrograms of polyA+ RNA were converted into double-stranded cDNA essentially as described in Kapranov et al, Science. 2007 Jun 8;316(5830):1484-8.
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| Hybridization protocol |
2 micrograms of double-stranded cDNA were labeled and hybridized to Affymetrix tiling arrays containing probes against non-repepetitive portions of human chromosomes 21 and 22 essentially as described in Kapranov et al, Science. 2007 Jun 8;316(5830):1484-8.
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| Scan protocol |
Arrays were scanned on Affymetrix GCS3000 scanner
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| Description |
single chip, 1 replicate
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| Data processing |
See supplement materials of Djebali, S, et al (2009) Nature in press
Number Sequences: 10, Data: signal, Scale: Linear, AlgName: TAS, AlgVersion: 1.1.01, probe_coordinate_convention: 0_indexed_center
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| Submission date |
Aug 11, 2009 |
| Last update date |
Aug 12, 2009 |
| Contact name |
Chris Zaleski |
| Organization name |
Cold Spring Harbor Labs
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| Lab |
Gingeras
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| Street address |
500 Sunnyside Blvd
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| City |
Woodbury |
| State/province |
NY |
| ZIP/Postal code |
11797 |
| Country |
USA |
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| Platform ID |
GPL5715 |
| Series (1) |
| GSE17600 |
Transcriptional termini of genes on chromosomes 21-22 (RACE mapping) |
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| Supplementary file |
Size |
Download |
File type/resource |
| GSM438379_PI_20_AS_BrCereb_Pool_7-10.cel.gz |
30.4 Mb |
(ftp)(http) |
CEL |
| GSM438379_PI_20_AS_BrCereb_Pool_7-10_signal.txt.gz |
9.2 Mb |
(ftp)(http) |
TXT |
| Processed data provided as supplementary file |
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