|
| Status |
Public on Jan 29, 2023 |
| Title |
SCC-25, tuni 6h rep1, HTA 2.0 |
| Sample type |
RNA |
| |
|
| Source name |
Human oral cancer cell line SCC28 treated with tunicamycin (5μg/ml)
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SCC25
treatment: tunicamycin
|
| Treatment protocol |
Cells were washed by PBS for 3 times, and added fresh medium containing tunicamycin (5μg/ml) or DMSO (0.1%) for 6 hours
|
| Growth protocol |
SCC25 cells were maiteined in DMEM/F12 medium with 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
RNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HTA 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Mar 06, 2020 |
| Last update date |
Jan 29, 2023 |
| Contact name |
Chenzhou Wu |
| E-mail(s) |
wuchenzhou@foxmail.com
|
| Organization name |
West china hospital of Stomatology, Sichuan university
|
| Street address |
Number 14, Unit 3, Renmin Nan Road,
|
| City |
Chengdu |
| ZIP/Postal code |
610041 |
| Country |
China |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE146505 |
Expression of mRNA and lncRNA in human oral cancer cell SCC25 treated with tunicamycin |
|
