|
| Status |
Public on Oct 06, 2023 |
| Title |
CD138+cells from normal_Replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow CD138+ cells,normal,replicate 1
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Normal
tissue: bone marrow
cell type: CD138+ cells
gender: Female
age: 59
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Bone marrow CD138+ cells were obtained from multiple myeloma patients and normal individuals by magnetic bead separation. The cells were treated with TRIzol to extract total RNA according to manufactures' instrctions.
|
| Label |
Cy3
|
| Label protocol |
mRNA was purified from total RNA after removal of rRNA (mRNA-ONLY™ Eukaryotic mRNA Isolation Kit, Epicentre). Then, each sample was amplified and transcribed into fluorescent cRNA along the entire length of the transcripts without 3' bias utilizing a random priming method (Arraystar Flash RNA Labeling Kit, Arraystar). The labeled cRNAs were purified by RNeasy Mini Kit (Qiagen). The concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000
|
| |
|
| Hybridization protocol |
1 μg of each labeled cRNA was fragmented by adding 5 μl 10 × Blocking Agent and 1 μl of 25 × Fragmentation Buffer, then heated the mixture at 60°C for 30 min, finally 25 μl 2 × GE Hybridization buffer was added to dilute the labeled cRNA. 50 μl of hybridization solution was dispensed into the gasket slide and assembled to the LncRNA expression microarray slide. The slides were incubated for 17 hours at 65°C in an Agilent Hybridization Oven.
|
| Scan protocol |
The hybridized arrays were washed, fixed and scanned with using the Agilent DNA Microarray Scanner (part number G2505C).
|
| Description |
lncRNA and mRMA expression data from bone marrow CD138+ cells of normal control 1
|
| Data processing |
Agilent Feature Extraction software (version 11.0.1.1) was used to analyze acquired array images. Quantile normalization and subsequent data processing were performed with using the GeneSpring GX v12.1 software package (Agilent Technologies).
|
| |
|
| Submission date |
Mar 10, 2020 |
| Last update date |
Oct 06, 2023 |
| Contact name |
Han Yan |
| E-mail(s) |
picachu122@126.com
|
| Organization name |
Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
|
| Street address |
Jiefang Avenue
|
| City |
Wuhan |
| ZIP/Postal code |
430022 |
| Country |
China |
| |
|
| Platform ID |
GPL21827 |
| Series (2) |
| GSE146757 |
Microarray analysis of mRNA and long noncoding RNA expression profiles between multiple myeloma patients and control |
| GSE159232 |
Multiple myeloma patients and control |
|
