|
| Status |
Public on Mar 01, 2023 |
| Title |
Ischemic gastrocnemius with proanthocyanidins treatment_adult mice_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Ischemic gastrocnemius
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: male
age: 10-12 weeks old
tissue: Ischemic gastrocnemius
treatment: proanthocyanidins
|
| Treatment protocol |
Six mice were anesthetized with 1% pentobarbital sodium solution (30 mg kg-1), and unilateral hindlimb ischemia was induced by femoral artery ligation. Then, those mice were divided into two groups randomly, they were treated via intraperitoneal injection daily from days 0 to 7 with with sterile water or proanthocyanidins. Mice were deeply anesthetized with pentobarbital sodium and then ischemic gastrocnemius were collected at days 7.
|
| Growth protocol |
Experimental animals were male and commercially-available C57BL/6 mice (Tengxin biotechnology co. LTD, Chongqing,China).
All mice were raised in Pathogen-free room and the room was maintained at a constant temperature of 23±1℃ with a 12 h light/dark cycle. Food and water were available ad libitum in the home cages.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol (Invitrogen) and miRNeasy mini kit (QIAGEN) according to manufacturer’s instructions, which efficiently recovered all RNA species, including miRNAs. RNA quality and quantity was measured by using nanodrop spectrophotometer (ND-1000, Nanodrop Technologies) and RNA Integrity was determined by gel electrophoresis
|
| Label |
Hy3
|
| Label protocol |
The miRCURY™ Hy3™/Hy5™ Power labeling kit (Exiqon, Vedbaek, Denmark) was used according to the manufacturer’s guideline for miRNA labelling
|
| |
|
| Hybridization protocol |
The Hy3TM-labeled samples were hybridized on the miRCURYTM LNA Array (v.19.0) (Exiqon) according to array manual
|
| Scan protocol |
The slides were scanned using the Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA).
|
| Description |
sample name: 177
|
| Data processing |
Scanned images were then imported into GenePix Pro 6.0 software (Axon) for grid alignment and data extraction. Replicated miRNAs were averaged and miRNAs that intensities>=50 in all samples were chosen for calculating normalization factor. Expressed data were normalized using the Median normalization. After normalization, differentially expressed miRNAs with statistical significance were identified through Volcano Plot filtering
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| |
|
| Submission date |
Mar 11, 2020 |
| Last update date |
Mar 01, 2023 |
| Contact name |
Du Chao |
| E-mail(s) |
a_do34@outlook.com
|
| Organization name |
The Affiliated Hospital of Southwest Medical University
|
| Street address |
25 Taiping Street, Jiangyang district
|
| City |
Luzhou city |
| ZIP/Postal code |
646000 |
| Country |
China |
| |
|
| Platform ID |
GPL28254 |
| Series (1) |
| GSE146772 |
miRNA expression profiling and identification of a miRNA regulator for muscle regeneration in the ischemic limb with proanthocyanidins treatment of adult mice |
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