|
| Status |
Public on Aug 12, 2010 |
| Title |
liver_feno_WT_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Liver, 0.2% fenofibrate (wt/wt) supplemented diet, wild type, 7 days
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Wild type FVB
tissue: Liver
age: 9 weeks old
gender: Male
|
| Treatment protocol |
At 8 weeks of age, a diet including 0.2% fenofibrate (wt/wt) was provided to mice for 7days. Then, mice were sacrified at age 9 weeks.
|
| Growth protocol |
Mice lacking phosphatidylcholine transfer protein (PC-TP) and wild type littermate controls on FVB/NJ background were maintained in a standard rodent diet 5001 (LabDiets) until 8 weeks of age.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from liver using Trizol according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the Affymetrix GeneChip Manual (rev 4.0) from 8ug total RNA.
|
| |
|
| Hybridization protocol |
20ug of cRNA was used for hybridization according to the Affymetrix GeneChip Manual, 45℃, overnight.
|
| Scan protocol |
GeneChips were scanned on an Affymetrix Model 3000 7G scanner with autoloader.
|
| Description |
none
|
| Data processing |
CEL files were loaded and analyzed in dChip. It was first normalized using an invariant normalization function. Normalized intensity values for individual genes expression were filtered out if a strong signal for the gene was not detected on greater than 30% of the GeneChips. Fold changes (Pctp-/- vs wild type) of at least 1.2 were considered to be significant for P < 0.05 by two-tailed Student’s t-Test.
|
| |
|
| Submission date |
Aug 14, 2009 |
| Last update date |
Aug 12, 2010 |
| Contact name |
hyewon kang |
| Organization name |
Brigham & Women's Hospital
|
| Street address |
75 Fransis street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE17666 |
Regulatory Role for PC-TP/StarD2 in the Metabolic Response to Peroxisome Proliferator Activated Receptor Alpha (PPARα) |
|
