|
| Status |
Public on Nov 26, 2020 |
| Title |
WBN4-5a |
| Sample type |
SRA |
| |
|
| Source name |
RNA derived from liquid culture in LB (OD600=0.4-0.6)
|
| Organism |
Pseudomonas aeruginosa |
| Characteristics |
strain: PA14 mutS::Tn
media: LB
culture number: 4
clone number: 5
replica: Replica 1
|
| Treatment protocol |
Directly after cultivation, the cells were treated with one volume of RNAprotect (Qiagen), incubated for 10 minutes at room temperature, centrifuged at 10.000 rpm and cell pellets were frozen at -80°C.
|
| Growth protocol |
Cells were grown in LB to an OD600 =0.4-0.6.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The RNeasy Mini Kit (Qiagen) in combination with Qiashredder columns (Qiagen) was used according to the manufacturer’s instruction.
The removal of ribosomal RNA was performed using the Ribo-Zero Bacteria Kit (Illumina) and cDNA libraries were generated with the ScriptSeq v2 Kit (Illumina).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
Single-colony-derived clone isolated from the 45th passage of WBN4 culture.
processed data file: ProcessedDataMatrix.xlsx
|
| Data processing |
Reads were trimmed for adaptor sequences and mapped to the PA14 whole genome using a Stampy pipeline with default parameters.
Genome_build: NC_008463.1 (PA14)
Supplementary_files_format_and_content: ProcessedDataMatrix.xlsx file is a data matrix that contains raw unique gene reads.
|
| |
|
| Submission date |
Mar 12, 2020 |
| Last update date |
Nov 26, 2020 |
| Contact name |
Susanne Häußler |
| E-mail(s) |
Susanne.Haeussler@helmholtz-hzi.de
|
| Organization name |
Twincore
|
| Department |
Molecular Bacteriology
|
| Street address |
Feodor-Lynen-Str. 7
|
| City |
Hanover |
| ZIP/Postal code |
30625 |
| Country |
Germany |
| |
|
| Platform ID |
GPL18287 |
| Series (1) |
| GSE146906 |
Adaptation of Pseudomonas aeruginosa to cultivation in standard laboratory conditions |
|
| Relations |
| BioSample |
SAMN14369339 |
| SRA |
SRX7904311 |
