|
| Status |
Public on Mar 12, 2022 |
| Title |
RCC4-siMESH1-1-repl2 |
| Sample type |
RNA |
| |
|
| Source name |
RCC4 transfected with MESH1 siRNA#2, biological rep2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: RCC4
|
| Treatment protocol |
RCC4 cells were transfected with indicated siRNAs for 48 hours
|
| Growth protocol |
RCC4 cells grew in the control full DMEM media supplement with 10% FBS and Penicillin/Streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted by RNAeasy kits (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA, hybridized to GeneChip® ____________ arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450
|
| |
|
| Hybridization protocol |
Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA, hybridized to GeneChip® ____________ arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450
|
| Scan protocol |
scanned with Affymetrix GeneChip® Scanner 7G according to standard Affymetrix GeneChip® Hybridization, Wash, and Stain protocols. (Affymetrix, Santa Clara,CA).
|
| Data processing |
The data were analyzed with Agilent 2100 Bioanalyzer G2939A
|
| |
|
| Submission date |
Mar 16, 2020 |
| Last update date |
Mar 13, 2022 |
| Contact name |
Jen-Tsan Chi |
| E-mail(s) |
jentsan.chi@duke.edu
|
| Phone |
919-668-4759
|
| Organization name |
Duke University
|
| Department |
Molecular Genetics and Microbiology
|
| Street address |
101 Science Drive, DUMC 3382
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27708 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE147062 |
MESH1 knockdown triggers proliferation arrest through TAZ repression |
|
