|
| Status |
Public on May 04, 2020 |
| Title |
PATU-8988T_DMSO_03 |
| Sample type |
SRA |
| |
|
| Source name |
PATU-8988T
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: PATU-8988T
cell type: pancreatic cancer cell line
treatment: DMSO
treatment time: 4h
|
| Treatment protocol |
Cells were plated on Day 0, treated 24 hours later with the indicated compound or DMSO for 4h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed in the dish using QIGEN Buffer RLT Plus. RNA was extracted using QIAGEN RNEasy Plus Mini Kit according to protocol.
RNA libraries were prepared for sequencing by Invitrogen
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Ion Torrent S5 |
| |
|
| Data processing |
Data processing of fastq files to counts was performed by Invitrogen using the Ion Torrent software suite.
Supplementary_files_format_and_content: The csv file contains mapped count values for each transcript for all samples in the study.
|
| |
|
| Submission date |
Mar 22, 2020 |
| Last update date |
May 04, 2020 |
| Contact name |
Zainab Murtaza Doctor |
| Organization name |
Dana Farber Cancer Institute
|
| Lab |
Gray Lab
|
| Street address |
360 Longwood Ave, LC-2106
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL23934 |
| Series (1) |
| GSE147340 |
Discovery and Characterization of a Potent and Selective Pin1 Inhibitor Targeted to an Active Cysteine Site |
|
| Relations |
| BioSample |
SAMN14421576 |
| SRA |
SRX7966059 |
