|
| Status |
Public on Mar 28, 2020 |
| Title |
Segfam low IMF mi 03_(miRNA-3_0) |
| Sample type |
RNA |
| |
|
| Source name |
MLD with low IMF content
|
| Organism |
Bos taurus |
| Characteristics |
breed: crossbred bull
age: 18 months
tissue: musculus longissimus dorsi (MLD)
imfcontent: low
|
| Treatment protocol |
assigment to either high or low IMF group
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Qiagen miReasy Mini kit TRI Reagent (Qiagen, Hilden, Germany) according to the manufacturer’s protocol for small-RNA. Then small RNA was cleaned up using the Rneasy MinElute Clean up kit (Qiagen, Hilden, Germany)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from miRNA according to FlashTag Biotin RNA Labeling Kit for Affymetrix GeneChip miRNA arrays (Genisphere, Hatfield, PA, USA) according to the manufacturer’s recommendations Affymetrix GeneChip miRNA Array protocols (Affymetrix, Santa Clara, CA, USA)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 42C on Affymetrix GeneChip miRNA arrays. GeneChips were washed and processed to detect biotin-containing transcripts by Streptavidin-PE (Phycoerythrin) conjugate.
|
| Scan protocol |
Scanning using the GeneChip scanner 3000 were performed according to Affymetrix protocols
|
| Description |
miRNA expression data in MLD from a bull with low IMF content
|
| Data processing |
Data were analysed with Expression Console and quantify by med polish
|
| |
|
| Submission date |
Mar 27, 2020 |
| Last update date |
Mar 28, 2020 |
| Contact name |
Steffen Maak |
| E-mail(s) |
maak@fbn-dummerstorf.de
|
| Organization name |
Leibniz Institute for Farm Animal Biology (FBN)
|
| Department |
Institute of Muscle Biology and Growth
|
| Lab |
Cellular Interactions
|
| Street address |
Wilhelm-Stahl-Allee 2, 18196 Dummerstorf
|
| City |
Rostock |
| State/province |
Mecklenburg-Vorpommern |
| ZIP/Postal code |
18196 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16384 |
| Series (1) |
| GSE147622 |
miRNA expression data from bulls with high and low intramusclar fat (IMF) content |
|
