|
| Status |
Public on Sep 09, 2009 |
| Title |
454 | R2Yaa IRF5+/- |
| Sample type |
protein |
| |
|
| Source name |
Murine antiserum, IRF5+/-
|
| Organism |
Mus musculus |
| Characteristics |
strain: R2Yaa IRF5+/-
|
| Biomaterial provider |
Renal Section, Dept. of Medicine, Boston University School of Medicine.
|
| Extracted molecule |
protein |
| Extraction protocol |
Whole antiserum was obtained.
|
| Label |
Cy3-conjugated goat anti-mouse antibody
|
| Label protocol |
Commercially available Cy3-conjugated goat anti-mouse antibody was added to the microarray at 1:4000 in 5% fetal bovine serum in phosphate-buffered saline with 0.1% Tween-20.
|
| |
|
| Hybridization protocol |
Murine antiserum was probed on the antigen microarray at 1:250 dilution in 5% fetal bovine serum in phosphate-buffered saline with 0.1% Tween-20, and incubated for 1.25 hours at 4 degrees Celsius.
|
| Scan protocol |
Fluorescent array images were collected for Cy3 with a GenePix 4000B fluorescent scanner at 10 micron resolution and image intensity data were extracted and analyzed with GenePix Pro 6.1 analysis software.
|
| Description |
Murine antiserum was obtained from blood collected from an individual mouse of the strain R2Yaa IRF5+/-. A 1:250 dilution of this antiserum was then applied onto the Utz Lab Whole Protein Autoantigen Array (V1.0), labeled with a goat anti-mouse Ig(M + G) secondary antibody conjugated to Cy3 (Jackson ImmunoResearch), and scanned with a GenePix 4000B scanner using GenePix Pro 6.1.
|
| Data processing |
After background correction and removal of flagged values, data was normalized by dividing each array value by the goat anti-mouse IgG (25 ug/ml) array feature signal intensity and then multiplying by 10,000 to obtain a normalized signal intensity value.
|
| |
|
| Submission date |
Aug 21, 2009 |
| Last update date |
Sep 08, 2009 |
| Contact name |
Chih Long Liu |
| Organization name |
Stanford University
|
| Department |
Medicine - Imm. & Rheum
|
| Lab |
Utz
|
| Street address |
269 Campus Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL9059 |
| Series (1) |
| GSE17926 |
Interferon regulatory factor 5 expression levels regulate murine lupus in a type-I interferon-independent manner |
|
