|
| Status |
Public on Aug 25, 2009 |
| Title |
Disc Cells 18 |
| Sample type |
RNA |
| |
|
| Source name |
Disc Cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: disc tissue
cell type: disc cells
treatment: Without TGF-B
|
| Treatment protocol |
Stem cells were treated with or without 10ng/nl TGF-B3.
|
| Growth protocol |
Cells were grown in a 3-D construct (Gelfoam, Pharmacia & Upjohn Co. Kalamazoo, MI) for 2-3 weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was harvested according to instructions with the Trizol isolation method.
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled cRNA were prepared according to the Affymetrix protocol for IVT labeling.
|
| |
|
| Hybridization protocol |
Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChips were scanned once via the Affymetrix 3000G scanner.
|
| Description |
Grown in 3-D construct.
|
| Data processing |
The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
|
| |
|
| Submission date |
Aug 24, 2009 |
| Last update date |
Aug 24, 2009 |
| Contact name |
Helen Gruber |
| E-mail(s) |
Helen.Gruber@carolinashealthcare.org
|
| Organization name |
Carolinas HealthCare System
|
| Department |
Orthopaedic Surgery
|
| Lab |
Orthopaedic Research
|
| Street address |
1542 Graden Terrace
|
| City |
Charlotte |
| State/province |
NC |
| ZIP/Postal code |
28203 |
| Country |
USA |
| |
|
| Platform ID |
GPL1352 |
| Series (1) |
| GSE17782 |
Gene Expression Data from Human Mesenchymal Stem Cells with and without TGF-B vs Human Annulus Disc Cells |
|
