|
| Status |
Public on Jun 30, 2020 |
| Title |
WM983BSPH |
| Sample type |
RNA |
| |
|
| Source name |
melamoma spheoid
|
| Organism |
Homo sapiens |
| Characteristics |
cell line source: WM983B metastatic melanoma cell line
cell type: melamoma spheoid
braf mutation status: V600E
|
| Treatment protocol |
Spheroid cultures were established by seeding 1.8x10^4 cells/well into Corning® Costar® Ultra-Low Attachment 6 plate. After 72 hours the medium was complemented with 10% FBS and the spheroids were grown for a week in complete medium. The visible spheroids were transfer then into a cell culture flask and leave them to attach.
|
| Growth protocol |
Human melanoma cell lines were maintained in RPMI 1640 medium supplemented with 10% foetal bovine serum, 300 mg/l L-glutamine and antibiotics. Resistant melanoma cell lines were maintained in the same medium containing PLX4720.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Qiagen Rneasy Plus Mini kit.
|
| Label |
biotin
|
| Label protocol |
Labeling, hybridization and image scan were performed according to the recent laboratory protocol of UD-GenoMed Medical Genomic Technologies Ltd.
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Data processing |
Affymetrix Expression Console was used for primary analysis, RMA algorithm with default settings.
|
| |
|
| Submission date |
Apr 14, 2020 |
| Last update date |
Jul 01, 2020 |
| Contact name |
Viktoria Koroknai |
| E-mail(s) |
koroknai.viktoria@med.unideb.hu
|
| Organization name |
University of Debrecen
|
| Department |
Department of Public Health and Epidemiology
|
| Street address |
26/B Kassai str.
|
| City |
Debrecen |
| ZIP/Postal code |
4028 |
| Country |
Hungary |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE148638 |
Expression data of BRAF inhibitor resistant melanoma cells and spheroids |
|
