|
Status |
Public on Feb 01, 2021 |
Title |
Lung, 026 |
Sample type |
SRA |
|
|
Source name |
Lung
|
Organism |
Macaca mulatta |
Characteristics |
gestational age at treatment: 130 days tx 1: LPS tx 1 route: Intra-amniotic injection tx 1 dose: 1mg tx 2: N/A tx2 route: N/A dose: N/A tx to delivery interval: 16 hours gestational age at delivery: 131 days tissue: Lung
|
Extracted molecule |
total RNA |
Extraction protocol |
total RNA was extracted with Trizol per manufacturer's guidelines. Afer polyA selection, paired-end RNA-sequencing with 75bp was done with a depth of 30M reads.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 3000 |
|
|
Data processing |
Alignment to the MMUL_10 done with Kallisto Gene summarization done with tximport Differential expression analysis was performed with DESeq2 Genome_build: MMUL_10
|
|
|
Submission date |
Apr 14, 2020 |
Last update date |
Feb 01, 2021 |
Contact name |
Augusto F Schmidt |
E-mail(s) |
aschmidt@med.miami.edu
|
Phone |
3052430156
|
Organization name |
University of Miami Miller School of Medicine
|
Street address |
1611 NW 12th ave
|
City |
Miami |
State/province |
FL |
ZIP/Postal code |
33136 |
Country |
USA |
|
|
Platform ID |
GPL23804 |
Series (1) |
GSE148645 |
Fetal lung maturation induced by chorioamnionitis and its interaction with antenatal corticosteroids in rhesus macaque fetuses |
|
Relations |
BioSample |
SAMN14596377 |
SRA |
SRX8114637 |