|
| Status |
Public on Feb 01, 2021 |
| Title |
Lung, 248 |
| Sample type |
SRA |
| |
|
| Source name |
Lung
|
| Organism |
Macaca mulatta |
| Characteristics |
gestational age at treatment: 126 days
tx 1: LPS
tx 1 route: Intra-amniotic injection
tx 1 dose: 1mg
tx 2: Betamethasone-acetate
tx2 route: Intramuscular injection
dose: 0.125mg/kg
tx to delivery interval: 5 days
gestational age at delivery: 131 days
tissue: Lung
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted with Trizol per manufacturer's guidelines.
Afer polyA selection, paired-end RNA-sequencing with 75bp was done with a depth of 30M reads.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Data processing |
Alignment to the MMUL_10 done with Kallisto
Gene summarization done with tximport
Differential expression analysis was performed with DESeq2
Genome_build: MMUL_10
|
| |
|
| Submission date |
Apr 14, 2020 |
| Last update date |
Feb 01, 2021 |
| Contact name |
Augusto F Schmidt |
| E-mail(s) |
aschmidt@med.miami.edu
|
| Phone |
3052430156
|
| Organization name |
University of Miami Miller School of Medicine
|
| Street address |
1611 NW 12th ave
|
| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33136 |
| Country |
USA |
| |
|
| Platform ID |
GPL23804 |
| Series (1) |
| GSE148645 |
Fetal lung maturation induced by chorioamnionitis and its interaction with antenatal corticosteroids in rhesus macaque fetuses |
|
| Relations |
| BioSample |
SAMN14596380 |
| SRA |
SRX8114650 |
