|
| Status |
Public on Apr 17, 2020 |
| Title |
05_ant_spik_r1 |
| Sample type |
SRA |
| |
|
| Source name |
sRNA Sorghum bicolor BTx623, spikelets with <0.5 mm anthers
|
| Organism |
Sorghum bicolor |
| Characteristics |
tissue: Spikelet
|
| Extracted molecule |
total RNA |
| Extraction protocol |
sRNA library constructed from all diverse tissue types listed in the field "source name" . The small RNA libraries were generated as described by Lu et al. (Methods 2007, 43:110), followed by Illumina sRNA TruSeq sample preparation kit followed by sequencing with Illumina's HiSeq 2500 instrument. The adapter sequences were removed, and the abundance of each distinct small RNA was determined.
|
| |
|
| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
5761
small RNA
|
| Data processing |
Small RNA-seq: the adapter sequences were trimmed from raw sequence reads, and then the small RNA sequences were trimmed to the lengths between 18 and 34 nucleotides.
Supplementary_files_format_and_content: Text containing distinct small RNAs with their raw abundance after removing the adapter sequences
|
| |
|
| Submission date |
Apr 16, 2020 |
| Last update date |
Sep 09, 2020 |
| Contact name |
Blake C. Meyers |
| E-mail(s) |
bmeyers@danforthcenter.org
|
| Phone |
314-587-1422
|
| Organization name |
Donald Danforth Plant Science Center
|
| Lab |
Meyers lab
|
| Street address |
975 N Warson Road
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63132 |
| Country |
USA |
| |
|
| Platform ID |
GPL21615 |
| Series (1) |
|
| Relations |
| BioSample |
SAMN14605347 |
| SRA |
SRX8123386 |
