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| Status |
Public on Nov 16, 2020 |
| Title |
Control_validation_3 |
| Sample type |
SRA |
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| Source name |
PBMC
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| Organism |
Homo sapiens |
| Characteristics |
type: Healthy control
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| Extracted molecule |
total RNA |
| Extraction protocol |
We extracted total RNA from PBMCs using TRIzol reagent (Invitrogen) and purified it with a mirVana miRNA Isolation Kit (Ambion, Thermo Fisher Scientific) according to the manufacturer’s protocol.
The sequencing libraries were constructed using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
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| Data processing |
Our newly developed pipeline, SPORTS1.0 (https://github.com/junchaoshi/sports1.0), was used to parse the raw sequencing data.
Genome_build: hg19
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| Submission date |
Apr 17, 2020 |
| Last update date |
Nov 16, 2020 |
| Contact name |
Musheng Li |
| E-mail(s) |
mushengl@unr.edu
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| Phone |
7753577859
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| Organization name |
University of Nevada, Reno
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| Street address |
North Virginia Street
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| City |
Reno |
| ZIP/Postal code |
89557 |
| Country |
USA |
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| Platform ID |
GPL11154 |
| Series (1) |
| GSE148862 |
Peripheral blood non-canonical small non-coding RNAs as novel biomarkers in lung cancer and pulmonary tuberculosis (validation cohort) |
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| Relations |
| BioSample |
SAMN14614051 |
| SRA |
SRX8132723 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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