|
| Status |
Public on Mar 07, 2021 |
| Title |
22Rv1+R24_2 |
| Sample type |
RNA |
| |
|
| Source name |
22Rv1 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: 22Rv1
cell type: prostate cancer cell line
arginine sensitivity: highly-sensitive
treatment: with arginine for 24hrs
|
| Treatment protocol |
22Rv1 cell line, highly-sensitive to arginine, was treated with or without arginine condition for 24 hours. PC3 cell line, sensitive to arginine, was treated with or without arginine condition for 48 hours.
|
| Growth protocol |
Both prostate cancer cell lines, 22Rv1 and PC3, are routinely cultured in RPMI-1640 medium with 10% FBS and 1% Pen/Strep.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was extracted by RNeasy Mini kit (Qiagen) with on-column DNase I digestion according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol.
|
| Scan protocol |
Standard Affymetrix protocol.
|
| Data processing |
The data were analyzed with Transcriptome Analysis Console (TAC) using Affymetrix default analysis settings.
|
| |
|
| Submission date |
Apr 27, 2020 |
| Last update date |
Mar 08, 2021 |
| Contact name |
Chia-Lin Chen |
| E-mail(s) |
truip75@gamil.com
|
| Organization name |
National Health Research Institutes
|
| Department |
Molecular and Genomic Medicine
|
| Lab |
Hsing-Jien Kung
|
| Street address |
No.35 Keyan Rd, Zhunan
|
| City |
Miaoli County |
| ZIP/Postal code |
35053 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE149427 |
Arginine effect on prostate cancer cell lines |
|
