|
| Status |
Public on Jul 31, 2012 |
| Title |
HSY3 |
| Sample type |
RNA |
| |
|
| Source name |
unstimulated primary murine B cell
|
| Organism |
Mus musculus |
| Characteristics |
agent: none
cell type: spleen B cell
age: 5 weeks
|
| Treatment protocol |
For B cell differentiation, purified murine B cell were treated with IL2, IL5, LPS for 24 hours.
|
| Growth protocol |
RPMI supplemented with 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNAeasy kit from Qiagen.
|
| Label |
digoxigenin
|
| Label protocol |
Standard ABI protocol, performed by the Emory Biomarker Core Center (http://www.biomarker.emory.edu/)
|
| |
|
| Hybridization protocol |
Applied Biosystem mouse genome survey
|
| Scan protocol |
Standard ABI protocol, performed by the Emory Biomarker Core Center (http://www.biomarker.emory.edu/)
|
| Description |
HSY3
|
| Data processing |
Sigle color cDNA microarray was used to analyze absoulte amount of RNA level from each sample. And Fold change data was used to reflects the level in IL2, IL5, LPS stimulated cell divided by control (unstimulated cell).
|
| |
|
| Submission date |
Sep 08, 2009 |
| Last update date |
Jul 31, 2012 |
| Contact name |
Jeremy Boss |
| E-mail(s) |
jmboss@emory.edu
|
| Organization name |
Emory University
|
| Department |
Micobiology and Immunology
|
| Lab |
Jeremy Boss
|
| Street address |
1510 Clifton Rd
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platform ID |
GPL2995 |
| Series (1) |
| GSE17999 |
ZBTB32 Is an Early Repressor of the CIITA MHC Class II Gene Expression during B Cell Differentiation to Plasma Cells |
|
