|
| Status |
Public on Jun 10, 2010 |
| Title |
Illumina: Rhesus macaque-superior frontal gyrus-26 years 28 days old |
| Sample type |
SRA |
| |
|
| Source name |
Dissected rhesus macaque post-mortem superior frontal gyrus
|
| Organism |
Macaca mulatta |
| Characteristics |
sex: female
age: 9518 day
tissue: superior frontal gyrus of the brain
batch: macaque batch 1
|
| Treatment protocol |
Rhesus macaque brains were obtained from the SuZhou Experimental Animal Center (SuZhou, China). The dissections were made from the cortical region approximately corresponding to Brodmann area 9 of the prefrontal cortex (the superior frontal gyrus). We aimed to make dissections that contain 2:1 grey:white matter (60-70% grey matter).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the frozen prefrontal cortex tissue using the Trizol (Invitrogen, USA) protocol with no modifications. Low molecular weight RNA was isolated, ligated to the adapters, amplified, and sequenced following the Small RNA preparation protocol (Illumina, USA) with no modifications.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
miRNA sequencing raw reads from post-mortem superior frontal gyrus of a 9518 day old human individual
|
| Data processing |
supplementary files contain small RNA sequences from purified low molecular weight RNA
|
| |
|
| Submission date |
Sep 08, 2009 |
| Last update date |
Jun 11, 2013 |
| Contact name |
Song Guo |
| Organization name |
PICB
|
| Street address |
320 Yue Yang Road
|
| City |
shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
|
| Platform ID |
GPL9160 |
| Series (2) |
| GSE18013 |
miRNA expression data from rhesus macaque postnatal brain in prefrontal cortex: time course |
| GSE18069 |
Gene and miRNA expression data from primate postnatal brain in prefrontal cortex: time course |
|
| Relations |
| BioSample |
SAMN02196559 |
