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Sample GSM454867 Query DataSets for GSM454867
Status Public on Jan 01, 2010
Title Male; 10d, rep1
Sample type RNA
 
Source name Pool 5 males (head & thorax only); 10d age
Organism Anopheles gambiae
Characteristics sex: Male
age: 10
Growth protocol Anopheles gambiae (strain G3) was reared under standard insectary conditions. Adults were given 10% sucrose solution ad libitum, however no blood meals were provided. Adults emerging within a 24h period were considered a single age class and each age class allocated to separate cages. Adult male and female mosquitoes were collected at 0 (≤ 24 h), 5, 10, 15, 20, 25 and 30 days post-eclosion. The abdomen was dissected and discarded. The heads and thoraces were stored pooled from 5 indivduals.
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy3
Label protocol All arrays were experiments were conducted acording to Agilent Technologies' One-colour Microarray-based Gene Expression Analysis protocol (version 5.7). 1 µg of total RNA and 5µl of 1:5000 dilution Agilent One-colour Spike-in mix were amplified and labelled using the Quick Amp Kit protocols (Agilent Technologies) following manufacturer's protocols.
 
Hybridization protocol Labelled cRNA was then purified and quantified before hybridization to the arrays using Agilent Technologies’ Gene Expression Hybridization Kit, SureHyb chambers, Microarray Hybridization oven and standard protocols. Hybridized arrays were washed with Agilent Gene Expression Wash Buffers (without the addition of Triton X102) and Stabilization and Drying Solution.
Scan protocol Microarrays were scanned and feature extraction undertaken by technicians at Oxford Gene Technology. Scans were at 5 µm resolution using the extended dynamic range feature of the Agilent Technologies’ DNA microarray scanner (G2505B)
Description none
Data processing Feature Extraction software (version 9.5.3.1; Agilent Technologies) was used to analyze scanned array image files according to Agilent’s One-colour Gene Expression protocol (GE1-v5_95_Feb07).
 
Submission date Sep 21, 2009
Last update date Sep 21, 2009
Contact name Steven Sinkins
E-mail(s) steven.sinkins@zoo.ox.ac.uk
Phone +44 (0) 1865 281047
Fax +44 (0) 1865 281545
Organization name University of Oxford
Department Department of Zoology
Street address South Parks Rd
City Oxford
ZIP/Postal code OX1 3PS
Country United Kingdom
 
Platform ID GPL9246
Series (1)
GSE18194 Transcriptional profiling across adult Anopheles gambiae age

Data table header descriptions
ID_REF
VALUE Normalized signal intensity was generated in GeneSpring GX as follows; threshold to 1.0, log2 transformation, 75th percentile shift normalization and baseline transformation to the median of all samples.

Data table
ID_REF VALUE
(-)3xSLv1 -0.8815298
(+)E1A_r60_1 -0.51278496
(+)E1A_r60_3 -0.7224059
(+)E1A_r60_a104 -0.50802994
(+)E1A_r60_a107 -0.41044283
(+)E1A_r60_a135 -0.44058418
(+)E1A_r60_a20 -0.3936906
(+)E1A_r60_a22 -0.2592907
(+)E1A_r60_a97 -0.38734055
(+)E1A_r60_n11 -0.41576958
(+)E1A_r60_n9 -0.3636651
(+)eQC-39 -0.9090028
(+)eQC-40 -0.92306757
(+)eQC-41 -0.9203639
(+)eQC-42 -0.82399416
CUST_1_PI378990374 3.0223894
CUST_10_PI378990374 -0.9045639
CUST_100_PI378990374 -0.037625313
CUST_1000_PI378990374 -0.99565697
CUST_10000_PI378990374 0.5211334

Total number of rows: 13314

Table truncated, full table size 433 Kbytes.




Supplementary file Size Download File type/resource
GSM454867_Uni_Ox_Cook_252030610002_S01_GE1-v5_95_Feb07_1_3.txt.gz 2.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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