|
Status |
Public on Sep 23, 2009 |
Title |
MC38 tumor PBS treated_29 |
Sample type |
RNA |
|
|
Source name |
MC38 tumor PBS treated
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 cell type: MC38 colon cancer cells mouse id number: 29 agent: PBS
|
Treatment protocol |
MC38 tumors in mice (~40 mm2) were injected with 50 micrograms of CpG1826 in 50 microliters PBS. Six hours later tumors were disected and frozen.
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Growth protocol |
MC38 colon cancer cells were grown in C57BL/6 mice subcutaneously
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using Qiagen RNAeasy kits
|
Label |
biotin
|
Label protocol |
Labelling & amplification kit used is the Affyemtrix WT (whole transcriptome) labelling and amplification kit
|
|
|
Hybridization protocol |
Hybridization was performed on Mouse Gene 1.0 ST arrays according to Affymetix instructions.
|
Scan protocol |
Affymetrix platform, using a fluidics station FS450 for washing and staining the arrays and a single laser GS-7000 scanner with autoloader. Expression Console software (Affymetrix) is used for preliminary data analysis and quality control
|
Description |
MC38 tumor PBS treated
|
Data processing |
The data were analyzed with R 2.7.1 using the affy and limma packages.
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|
|
Submission date |
Sep 22, 2009 |
Last update date |
Sep 22, 2009 |
Contact name |
Michael Henry Kershaw |
E-mail(s) |
michael.kershaw@petermac.org
|
Phone |
(613) 96561177
|
Fax |
(613) 96561411
|
Organization name |
Peter MacCallum Cancer Centre
|
Department |
Research
|
Lab |
Cancer Immunotherapy
|
Street address |
14 St. Andrews Place
|
City |
Melbourne |
State/province |
Victoria |
ZIP/Postal code |
3002 |
Country |
Australia |
|
|
Platform ID |
GPL6246 |
Series (1) |
GSE18203 |
Intratumoral injection of CpG1826 |
|