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Sample GSM455163 Query DataSets for GSM455163
Status Public on Sep 23, 2009
Title MC38 tumor PBS treated_482
Sample type RNA
 
Source name MC38 tumor PBS treated
Organism Mus musculus
Characteristics strain: C57BL/6
cell type: MC38 colon cancer cells
mouse id number: 482
agent: PBS
Treatment protocol MC38 tumors in mice (~40 mm2) were injected with 50 micrograms of CpG1826 in 50 microliters PBS. Six hours later tumors were disected and frozen.
Growth protocol MC38 colon cancer cells were grown in C57BL/6 mice subcutaneously
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Qiagen RNAeasy kits
Label biotin
Label protocol Labelling & amplification kit used is the Affyemtrix WT (whole transcriptome) labelling and amplification kit
 
Hybridization protocol Hybridization was performed on Mouse Gene 1.0 ST arrays according to Affymetix instructions.
Scan protocol Affymetrix platform, using a fluidics station FS450 for washing and staining the arrays and a single laser GS-7000 scanner with autoloader. Expression Console software (Affymetrix) is used for preliminary data analysis and quality control
Description MC38 tumor PBS treated
Data processing The data were analyzed with R 2.7.1 using the affy and limma packages.
 
Submission date Sep 22, 2009
Last update date Sep 22, 2009
Contact name Michael Henry Kershaw
E-mail(s) michael.kershaw@petermac.org
Phone (613) 96561177
Fax (613) 96561411
Organization name Peter MacCallum Cancer Centre
Department Research
Lab Cancer Immunotherapy
Street address 14 St. Andrews Place
City Melbourne
State/province Victoria
ZIP/Postal code 3002
Country Australia
 
Platform ID GPL6246
Series (1)
GSE18203 Intratumoral injection of CpG1826

Data table header descriptions
ID_REF
VALUE RMA normalized log base 2 signal intesity.

Data table
ID_REF VALUE
10338001 11.91681249
10338003 10.71433854
10338004 9.512601443
10338017 12.65628181
10338025 9.259976461
10338026 13.20713153
10338029 9.669136784
10338035 9.315625639
10338036 9.736541567
10338037 5.147495065
10338041 10.89770469
10338042 10.43150728
10338044 11.95604093
10338047 8.129101182
10338056 3.542617815
10338059 13.20688748
10338060 5.456967218
10338063 4.362381273
10338064 7.198274298
10338065 8.113423753

Total number of rows: 35512

Table truncated, full table size 724 Kbytes.




Supplementary file Size Download File type/resource
GSM455163.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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