|
| Status |
Public on Sep 23, 2009 |
| Title |
MC38 tumor PBS treated_592 |
| Sample type |
RNA |
| |
|
| Source name |
MC38 tumor PBS treated
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: MC38 colon cancer cells
mouse id number: 592
agent: PBS
|
| Treatment protocol |
MC38 tumors in mice (~40 mm2) were injected with 50 micrograms of CpG1826 in 50 microliters PBS. Six hours later tumors were disected and frozen.
|
| Growth protocol |
MC38 colon cancer cells were grown in C57BL/6 mice subcutaneously
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen RNAeasy kits
|
| Label |
biotin
|
| Label protocol |
Labelling & amplification kit used is the Affyemtrix WT (whole transcriptome) labelling and amplification kit
|
| |
|
| Hybridization protocol |
Hybridization was performed on Mouse Gene 1.0 ST arrays according to Affymetix instructions.
|
| Scan protocol |
Affymetrix platform, using a fluidics station FS450 for washing and staining the arrays and a single laser GS-7000 scanner with autoloader. Expression Console software (Affymetrix) is used for preliminary data analysis and quality control
|
| Description |
MC38 tumor PBS treated
|
| Data processing |
The data were analyzed with R 2.7.1 using the affy and limma packages.
|
| |
|
| Submission date |
Sep 22, 2009 |
| Last update date |
Sep 22, 2009 |
| Contact name |
Michael Henry Kershaw |
| E-mail(s) |
michael.kershaw@petermac.org
|
| Phone |
(613) 96561177
|
| Fax |
(613) 96561411
|
| Organization name |
Peter MacCallum Cancer Centre
|
| Department |
Research
|
| Lab |
Cancer Immunotherapy
|
| Street address |
14 St. Andrews Place
|
| City |
Melbourne |
| State/province |
Victoria |
| ZIP/Postal code |
3002 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE18203 |
Intratumoral injection of CpG1826 |
|
