|
| Status |
Public on May 21, 2020 |
| Title |
Sorghum spikelet <0.5 mm anthers |
| Sample type |
SRA |
| |
|
| Source name |
spikelet
|
| Organism |
Sorghum bicolor |
| Characteristics |
sample type: Sorghum spikelet <0.5 mm anthers
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol RNA
The library construction is based on a PARE protocol modified by Sandra Mathioni and used the Illumina TruSeq sRNA Indexes for the 3 prime adapter. The PARE library was constructed by Sandra Mathioni at the Donald Danforth Plant Science Center and sequenced on an Illumina HiSeq 2500 at the University of Delaware.
PARE-end
|
| |
|
| Library strategy |
OTHER |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
PARE
|
| Data processing |
PARE-seq: the adapter sequences were trimmed from raw sequence reads, and then the small RNA sequences were trimmed to the lengths between 18 and 20 nucleotides.
Supplementary_files_format_and_content: Tag counts
|
| |
|
| Submission date |
May 20, 2020 |
| Last update date |
May 21, 2020 |
| Contact name |
Alex Harkess |
| E-mail(s) |
aharkess@hudsonalpha.org
|
| Organization name |
HudsonAlpha Institute for Biotechnology
|
| Street address |
601 Genome Way
|
| City |
Huntsville |
| State/province |
AL |
| ZIP/Postal code |
35806 |
| Country |
USA |
| |
|
| Platform ID |
GPL21615 |
| Series (1) |
| GSE150798 |
Sorghum bicolor and Setaria viridis PARE-seq |
|
| Relations |
| BioSample |
SAMN14983968 |
| SRA |
SRX8370408 |
