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| Status |
Public on Jun 23, 2020 |
| Title |
NAM_1d S Rep 4 |
| Sample type |
SRA |
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| Source name |
Spleen
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| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
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| Treatment protocol |
0.5% NAM was added to the drinking water of treated animals. NAM was replaced every 7 days.
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| Growth protocol |
NA
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| Extracted molecule |
polyA RNA |
| Extraction protocol |
Lymph nodes and spleens were removed from the mice and RNA freshly extracted using Qiagen RNeasy kits.
mRNA libraries were prepared using the Truseq mRNA library prep kit (Illumina) and sequenced in paired end mode (2X75)
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
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| Description |
S19
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| Data processing |
Sequencing, data quality, reads repartition (e.g., for potential ribosomal contamination), and insert size estimation were performed using FastQC, Picard-Tools, Samtools and rseqc
Reads were mapped using STAR_2.4.0
Genome_build: mm10
Supplementary_files_format_and_content: Tab-delimited text file includes deseq2 normalized expression values for each gene and for each sample. Annotations from Mouse FAST DB v2016_1 were also provide
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| Submission date |
May 21, 2020 |
| Last update date |
Jun 23, 2020 |
| Contact name |
Pierre de la Grange |
| E-mail(s) |
pierre.delagrange@genosplice.com
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| Organization name |
GenoSplice technology
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| Department |
Paris Biotech Santé
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| Street address |
29, rue du Faubourg Saint-Jacques
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| City |
Paris |
| ZIP/Postal code |
75014 |
| Country |
France |
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| Platform ID |
GPL19057 |
| Series (2) |
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| Relations |
| BioSample |
SAMN14987705 |
| SRA |
SRX8374107 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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