|
| Status |
Public on Aug 21, 2020 |
| Title |
batch1_JH_B7_15 |
| Sample type |
RNA |
| |
|
| Source name |
lung tissue
|
| Organism |
Homo sapiens |
| Characteristics |
patient: 6970
age: 55
disease state: COPD
tissue: transplanted lung tissue
|
| Treatment protocol |
Not Applicable.
|
| Growth protocol |
Not Applicable.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
“RNeasy Mini” tool kit from Qiagen was applied to extract the total RNA in this study
|
| Label |
Biotin
|
| Label protocol |
The fragmented cDNA is labeled by terminal deoxynucleotidyl transferase (TdT) using a proprietary DNA Labeling Reagent that is covalently linked to biotin 5.5μg of single-stranded cDNA is required for fragmentation and labeling.
|
| |
|
| Hybridization protocol |
GeneChip™ Hybridization, Wash, and Stain Kits were applied in this study.
|
| Scan protocol |
GeneChip™ Scanner 3000 System was utilized to scan the arrays in this study.
|
| Data processing |
The analysis tools, including robust multichip array (RMA) algorithm in “affy” (v1.36.1) package and the BrainArray v17 CDF in R (version v3.3.0), were applied to analyze the Microarray expression profile
After the above standard data processing, the normalized expression matrix was log2 transformed to get the final expression matrix
|
| |
|
| Submission date |
May 22, 2020 |
| Last update date |
Aug 22, 2020 |
| Contact name |
FENG XU |
| E-mail(s) |
feng.xu@hli.ubc.ca
|
| Phone |
6047818321
|
| Organization name |
UBC
|
| Department |
Department of Pathology and Lab Medicine
|
| Lab |
Jim Hogg Lab
|
| Street address |
Suite 110, 6309 Larkin Dr, 110
|
| City |
VANCOUVER |
| State/province |
BC |
| ZIP/Postal code |
V6T 0C2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL17556 |
| Series (1) |
| GSE151052 |
The site and nature of terminal bronchiolar destruction in chronic obstructive pulmonary disease (COPD). |
|
